Characterization of neuraminidases produced by various serotypes of Pasteurella haemolytica
نویسندگان
چکیده
منابع مشابه
Characterization of neuraminidases produced by various serotypes of Pasteurella multocida.
Neuraminidases produced by 16 strains of Pasteurella multocida (serotypes 1 to 16) were characterized by molecular weight, substrate specificity, and antigenic identity. After growth in a chemically defined medium, stage I (lyophilized) culture supernatants were assayed for activity with N-acetylneuramin lactose, human alpha-1-acid glycoprotein, fetuin, colominic acid, and bovine submaxillary m...
متن کاملCharacterization of lipopolysaccharides from four Pasteurella haemolytica serotype strains: evidence for presence of sialic acid in serotypes 1 and 5.
Highly purified lipopolysaccharides (LPS) obtained from four strains of Pasteurella haemolytica representative of four different serotypes were studied to ascertain their overall structural elements and sugar and fatty acid compositions. SDS-PAGE analysis revealed that each LPS was of the smooth-type although they differed in migration patterns. Somewhat unusual features of these LPS included t...
متن کاملPulmonary Endothelial Cells by Pasteurella haemolytica Leukotoxin
In this study, we used an in vitro coculture system to determine which virulence factor from Pasteurella haemolytica Al was responsible for augmenting bovine polymorphonuclear neutrophil (PMN)-mediated killing of bovine pulmonary artery endothelial cells (BPAEC). A 5"Cr release cytotoxicity assay was used as a measure of BPAEC killing. The mechanisms associated with this BPAEC killing were also...
متن کاملPasteurella haemolytica A2
Twenty-one healthy local goats of about eight months old were divided into four groups consisting of six animals in groups 1, 2 and 3 and three animals in group 4. Goats in groups 1 and 2 were inoculated intratracheally with Mycoplasma arginini. Goats in group 2 were inoculated again with Pasteurella haemolytica A2 six days later. Goats in group 3 were inoculated intratracheally with P. haemoly...
متن کاملMolecular analysis of the leukotoxin determinants from Pasteurella haemolytica serotypes 1 to 16.
All sixteen serotypes of Pasteurella haemolytica were shown to produce a leukotoxin protein which is immunologically related to the well-characterized serotype 1 leukotoxin. All of the leukotoxins were weakly hemolytic and were able to bind to BL-3 target cells. The leukotoxin determinants were characterized by Southern blot hybridization by use of the previously cloned serotype 1 determinant a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1993
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.61.11.4669-4674.1993